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Andris Abramenkovs - Field Application Scientist, Nordics

At present, flow cytometry is the most rapid method for detection of DSBs and cell viability. In this chapter, we provide our experience and methodological modification of flow cytometry protocol for the detection of γ-H2AX, a well-known marker of DSBs, in fixed mammalian fibroblasts. 2018-06-01 · Reportedly the gamma-H2AX can be quantified either by immunoflourescence or flow cytometry . Studies have suggested gamma H2AX quantification by immunoflourescence as a useful biomarker of human low level radiation exposure. In immunofluorescence method numbers of foci formed are individually counted by microscopic evaluation. This process is believed to play a key role in the repair of DNA damage. In this study, we established a flow cytometry (FCM) system for measuring radiation-induced phosphorylated histone H2AX (gammaH2AX) in cultured human T lymphocytes to evaluate individual radiation sensitivity in vitro.

In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations. To this end, we selected 14 well-known genotoxic compounds and compared them with 10 nongenotoxic chemicals, using CHO-9 cells because they are well characterized as to DNA repair and DDR. We quantified gamma-H2AX foci manually and automatically. In addition, total gamma-H2AX activation was determined by flow cytometry. 2021-03-15 · View This Abstract Online; Flow cytometric detection of gamma-H2AX to evaluate DNA damage by low dose diagnostic irradiation.

Targeting HSP90 with the inhibitor AT13387 in tumour cells - PDF

Ex vivo uniformly X-irradiated blood lymphocytes from one donor were used to generate a universal biexponential calibration function for γ-H2AX foci/intensity yields per unit dose for time points up to 96 hours post exposure. 2010-02-04 · Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 2008; 36 : 5678–5694. CAS PubMed PubMed Central Google Scholar 2015-06-11 · This suggests that, in line with the low residual slope observed in the saturation region with flow cytometry, there is still room for further H2AX phosphorylation at such high doses.

Cell Cycle-specific Measurement of γH2AX and Apoptosis

Several reports show that the level of gamma-H2AX as detected by flow cytometry correlates well with the number of DNA strand breaks, to the level of cell death and radiosensitivity (32–34).

Products (63) User Reviews (2) Company View. Product View. Your search returned 63 H2AX Flow Cytometry Antibodies across 9 suppliers. Showing 9 of 9 suppliers (63 products total) <<. Histone H2AX: Products.
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Showing 9 of 9 suppliers (63 products total) <<. Histone H2AX: Products. Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes.

P-H2AX can be measured by flow cytometry or counted in separate cell nuclei flow cytometry may be well suited for measurements of the P-H2AX response in av MG till startsidan Sök — Validation of a flow cytometry-based detection of γ-H2AX, to measure DNA damage for clinical applications. Cytometry: Part B - Clin Cytometry Microwaves from GSM mobile telephones affect 53BP1 and gamma-H2AX foci in human of efflux pumps as determined by FUN-1 staining and flow cytometry. Validation of a flow cytometry-based detection of γ-H2AX, to measure DNA A flow cytometry assay that measures cellular sensitivity to DNA-damaging agents, Furthermore, the effect of hypothermia was investigated at the level of phosphorylated histone 2AX (gamma H2AX) foci, clonogenic cell survival and micronuclei not genotoxic in a flow cytometry-based micronucleus assay in vivo2013Ingår Involvement of CYP1B1 in interferon gamma-induced alterations of epithelial using Pig-a and flow cytometric micronucleus assays.
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Targeting HSP90 with the inhibitor AT13387 in tumour cells - PDF

Histone H2AX: Products.

Andris Abramenkovs - Field Application Scientist, Nordics

Several reports show that the level of gamma-H2AX as detected by flow cytometry correlates well with the number of DNA strand breaks, to the level of cell death and radiosensitivity ( 32–34 ). At present, flow cytometry is the most rapid method for detection of DSBs and cell viability. In this chapter, we provide our experience and methodological modification of flow cytometry protocol for the detection of γ-H2AX, a well-known marker of DSBs, in fixed mammalian fibroblasts.

Flow Cytometry-Based Gamma-H2AX Assay for Radiation Biodosimetry protocol (method) by Ghazi Alsbeih Anti-H2AX Flow Cytometry Antibody Products. Anti-H2AX Flow Cytometry Antibody Products. Products (63) User Reviews (2) Company View. Product View. Your search returned 63 H2AX Flow Cytometry Antibodies across 9 suppliers.